Ottawa says it is imposing economic sanctions on 16 more Russian "entities" over that country's actions in Ukraine.
Prime Minister Stephen Harper issued a statement Sunday saying Russia's "illegal occupation of Ukraine and provocative military activity" remains a serious concern.
Harper says the latest measures should increase the economic pressure on Russia and "those responsible for the crisis in Ukraine."
In the last couple of months, the Harper government has imposed a number of political and economic sanctions against senior officials and some institutions in Russia and Ukraine.
Ottawa has expelled a Russian diplomat, co-sponsored a UN resolution on the crisis and earmarked $220 million to help stabilize the Ukrainian economy.
Canada has also said it will send six CF-18 fighter-bombers to join a NATO air police mission.
"We remain committed to working with our allies to preserve and promote a free, democratic and peaceful world, and will take further actions if Ukraine's sovereignty and territorial integrity continues to be threatened," Harper said.