Police fired tear gas at rowdy fans at Marseille's Old Port for the second straight day on Friday.
The outburst of violence came despite heavy police presence a day before England is to play Russia at the European Championship in the Mediterranean port city's Stade Velodrome.
There were no immediate reports of injuries.
When the first round of tear gas cleared, hundreds of England fans remained in the area, singing and waving English flags as riot police stood nearby, watching closely.
When a car with flashing police lights drove by at one point in the evening, a fan threw a beer bottle at the vehicle.
Police later ran into the crowd and grabbed a man in a red England shirt, handcuffing him and leading him away.
Things calmed a bit after the man was taken away, but police soon fired more tear gas at the England fans.
About a block away, people sat on terraces outside restaurants eating.
Earlier Friday, cleaners hosed down sidewalks and swept up broken glass left over from Thursday's incidents.
The manager of an Irish bar near where soccer supporters from England fought overnight with locals in Marseille's historic centre said youths from the city's gritty suburbs provoked the clashes.
The brief clashes have revived bitter memories of days of bloody fighting in this Mediterranean port city between England hooligans, Tunisia fans and locals of North African origin during the World Cup in 1998, and raised fears of more violence ahead of Saturday's match.
French Interior Ministry spokesman Pierre-Henry Brandet said four police officers were lightly hurt in Thursday's violence and police detained two people, one French and one English.
"UEFA regrets the skirmishes that occurred in Marseille yesterday," the governing body of European soccer said Friday in a statement. "We are confident that the safety of travelling fans will be ensured by the local authorities which are responsible for order in the city. We make an appeal to all fans to behave respectfully throughout the tournament."
Following deadly attacks in Paris by Islamic extremists and fears Euro 2016 is also a target, security already is at an unprecedented high for the month-long tournament that kicks off Friday in Paris when host France takes on Romania.
Anthony Heraud, the 34-year-old manager of Irish pub O'Malley's, told The Associated Press that local youths started the trouble on Thursday in the Old Port.
"There were some small exchanges but nothing too nasty," Heraud said. "Englishmen are cool. They were just partying, singing a lot. But no problem."
An Associated Press reporter saw fans throwing bottles and plastic chairs at one another. Riot police with shields separated the two groups and fired tear gas to disperse them.
Mark Roberts, head of soccer policing in Britain, said England fans had been in the city "without issue" until locals arrived.
"At around midnight, there was a short confrontation where a group of approximately 70 local youths approached a pub where England fans had congregated," Roberts said. "This was quickly dealt with by French police and one English supporter was arrested. We are aware of no further incidents overnight."
At about noon on Friday, several white minibuses full of police were seen heading toward the Old Port.
Heraud said he hoped that beefed-up security would rein in potential violence in the coming 48 hours and prevent a repeat of the ugly scenes of the 1998 World Cup.
Then, hundreds of England fans were involved in violent beach-front clashes with locals in Marseille over two days around England's match against Tunisia match, prompting a headline in a local paper: "Go home hooligans!"
One fan had his throat slashed, cafe windows were smashed, and bottles, glasses and chairs were thrown. The scenes further marred the reputation of England's soccer fans following the dark days of hooliganism in the 1970s and '80s.
English soccer fans have been largely praised for their behaviour during recent tournaments.